recombinant mouse cd73 Search Results


murine cd73 mcd73 proteins  (TargetMol)
93
TargetMol murine cd73 mcd73 proteins
Murine Cd73 Mcd73 Proteins, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+cd73/CD73-5'-Nucleotidase+Protein%2C+Mouse%2C+Recombinant/pm41265550-119-6-13
Average 93 stars, based on 1 article reviews
murine cd73 mcd73 proteins - by Bioz Stars, 2026-11
93/100 stars
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cd73 +hucmsc-evs  (Baiao Pharmaceuticals)
90
Baiao Pharmaceuticals cd73 +hucmsc-evs
Characteristics of hucMSC-EVs and <t>CD73</t> +hucMSC-EVs. A Representative images of hucMSCs and CD73+ hucMSCs are observed under microscopy. B Representative images of hucMSC-EVs and CD73+ hucMSC-EVs are observed under transmission electron microscopy (TEM). C Size distribution of extracellular vesicle is measured by nanoparticle tracking analysis (NTA, ZetaView, Particle Metrix Inc., German). D Western-blotting analysis of indicated proteins is detected, including the modified protein of CD73, exosomal positive biomarkers of CD9, CD63, CD81, TSG101, and ALIX, and exosomal negative biomarker of calnexin. (E and F) The ATP and AMP hydrolytic activities of the indicated dose of hucMSC-EVs or CD73+hucMSC-EVs with or without 100 μM APCP are measured in vitro (n = 3)
Cd73 +Hucmsc Evs, supplied by Baiao Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+cd73/recombinant+mouse+cd73/pmc08425042-104-35-50
Average 90 stars, based on 1 article reviews
cd73 +hucmsc-evs - by Bioz Stars, 2026-11
90/100 stars
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recombinant mouse 5'-nucleotidase/cd73/nt5e  (Beijing Solarbio Science)
N/A
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recombinant mouse 5'-nucleotidase/cd73 protein, cf  (R&D Systems)
N/A
Recombinant Mouse 5'-Nucleotidase/CD73 Protein, CF
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recombinant mouse cd73 nt5e protein  (Elabscience)
N/A
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recombinant mouse 5 nucleotidase cd73 protein  (Novus Biologicals)
N/A
The Recombinant Mouse 5 Nucleotidase CD73 Protein has been validated for the following applications Functional SDS Page
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recombinant mouse 5 nucleotidase cd73 protein cf  (R&D Systems)
N/A
The Recombinant Mouse 5 Nucleotidase CD73 Protein from R D Systems is derived from CHO The Recombinant Mouse 5 Nucleotidase CD73 Protein has been validated for the following applications Enzyme Activity
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rat recombinant anti-mouse cd73 recombinant antibody  (Angio-Proteomie)
N/A
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5'-nucleotidase ecto (cd73) mouse recombinant  (Angio-Proteomie)
N/A
NT5E Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 531 amino acids (29-551a.a.) and having a molecular mass of 59.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).NT5E
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cd73, avi-his-tag (mouse) recombinant  (BPS Bioscience)
N/A
Recombinant mouse CD73 (5'-nucleotidase), encompassing amino acids 29-549. This construct contains a C-terminal Avi-His-tag (6xHis). This recombinant protein was affinity purified.
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mouse 5'-nucleotidase/cd73 recombinant protein  (Boster Bio)
N/A
Mouse 5'-Nucleotidase/CD73 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 29-551aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
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cd73, avi-his-tag, biotin-labeled (mouse) recombinant  (BPS Bioscience)
N/A
Recombinant mouse CD73 (5'-nucleotidase), encompassing amino acids 29-549 and contains a C-terminal Avi-His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
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Image Search Results


Characteristics of hucMSC-EVs and CD73 +hucMSC-EVs. A Representative images of hucMSCs and CD73+ hucMSCs are observed under microscopy. B Representative images of hucMSC-EVs and CD73+ hucMSC-EVs are observed under transmission electron microscopy (TEM). C Size distribution of extracellular vesicle is measured by nanoparticle tracking analysis (NTA, ZetaView, Particle Metrix Inc., German). D Western-blotting analysis of indicated proteins is detected, including the modified protein of CD73, exosomal positive biomarkers of CD9, CD63, CD81, TSG101, and ALIX, and exosomal negative biomarker of calnexin. (E and F) The ATP and AMP hydrolytic activities of the indicated dose of hucMSC-EVs or CD73+hucMSC-EVs with or without 100 μM APCP are measured in vitro (n = 3)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: Characteristics of hucMSC-EVs and CD73 +hucMSC-EVs. A Representative images of hucMSCs and CD73+ hucMSCs are observed under microscopy. B Representative images of hucMSC-EVs and CD73+ hucMSC-EVs are observed under transmission electron microscopy (TEM). C Size distribution of extracellular vesicle is measured by nanoparticle tracking analysis (NTA, ZetaView, Particle Metrix Inc., German). D Western-blotting analysis of indicated proteins is detected, including the modified protein of CD73, exosomal positive biomarkers of CD9, CD63, CD81, TSG101, and ALIX, and exosomal negative biomarker of calnexin. (E and F) The ATP and AMP hydrolytic activities of the indicated dose of hucMSC-EVs or CD73+hucMSC-EVs with or without 100 μM APCP are measured in vitro (n = 3)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: Microscopy, Transmission Assay, Electron Microscopy, Western Blot, Modification, Biomarker Discovery, In Vitro

CD73+ ucMSC-EVs promote M2 polarization and inhibit M1 polarization in vitro. A The effects of 30 ng/ml CD73, 30 μg/ml hucMSC-EVs and 30 μg/ml CD73+hucMSC-EVs with or without 100 μM APCP on the change of M1 subsets in BV2 cells treated with 1 μg/ml LPS are determined by immunofluorescences. B The effects of 30 ng/ml CD73, 30 μg/ml hucMSC-EVs and 30 μg/ml CD73+ hucMSC-EVs with or without 100 μM APCP on the change of M2 subsets in BV2 cells treated with 5 μg/ml IL-4 are determined by immunofluorescences. C and D Fluorescent intensities are normalized to fluorescent levels in LPS group or IL-4 group (*p < 0.05 versus LPS/IL-4, #p < 0.05 versus LPS/IL-4 + CD73+hucMSC-EVs, n = 3)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: CD73+ ucMSC-EVs promote M2 polarization and inhibit M1 polarization in vitro. A The effects of 30 ng/ml CD73, 30 μg/ml hucMSC-EVs and 30 μg/ml CD73+hucMSC-EVs with or without 100 μM APCP on the change of M1 subsets in BV2 cells treated with 1 μg/ml LPS are determined by immunofluorescences. B The effects of 30 ng/ml CD73, 30 μg/ml hucMSC-EVs and 30 μg/ml CD73+ hucMSC-EVs with or without 100 μM APCP on the change of M2 subsets in BV2 cells treated with 5 μg/ml IL-4 are determined by immunofluorescences. C and D Fluorescent intensities are normalized to fluorescent levels in LPS group or IL-4 group (*p < 0.05 versus LPS/IL-4, #p < 0.05 versus LPS/IL-4 + CD73+hucMSC-EVs, n = 3)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: In Vitro

CD73+ ucMSC-EVs augment M2/M1 polarization via A 2b adenosine receptor activation. A and B BV2 cells are treated with 1 μg/ml LPS in the presence or absence of 30 μg/ml CD73+hucMSC-EVs, and together with 1 μM MRS1706 (A 2b R inhibitor) or 1 μM SCH58261 (A 2a R inhibitor). A Intracellular cAMP levels are measured after 10 min stimulation (n = 5). B PKA protein expression in BV2 cells is detected (n = 3). (* p < 0.05 versus control, #p < 0.05 versus CD73+ hucMSC-EVs) C – F BV2 cells are treated with 1 μg/ml LPS or 5 μg/ml IL-4 in the presence or absence of 30 μg/ml CD73+hucMSC-EVs, and together with the indicated dose of MRS1706 or SCH58261. The iNOS/Arg-1 protein expression in BV2 cells was either investigated. G The mRNA relative expression level of M1 phase is detected, including TNF-α, IL-1β, iNOS, and CD86. H The mRNA relative expression level of M2 phase is detected, including arginase 1, IL-10, and CD206. (* p < 0.05 versus LPS/IL-4, #p < 0.05 versus LPS/IL-4 + CD73+hucMSC-EVs, n = 3)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: CD73+ ucMSC-EVs augment M2/M1 polarization via A 2b adenosine receptor activation. A and B BV2 cells are treated with 1 μg/ml LPS in the presence or absence of 30 μg/ml CD73+hucMSC-EVs, and together with 1 μM MRS1706 (A 2b R inhibitor) or 1 μM SCH58261 (A 2a R inhibitor). A Intracellular cAMP levels are measured after 10 min stimulation (n = 5). B PKA protein expression in BV2 cells is detected (n = 3). (* p < 0.05 versus control, #p < 0.05 versus CD73+ hucMSC-EVs) C – F BV2 cells are treated with 1 μg/ml LPS or 5 μg/ml IL-4 in the presence or absence of 30 μg/ml CD73+hucMSC-EVs, and together with the indicated dose of MRS1706 or SCH58261. The iNOS/Arg-1 protein expression in BV2 cells was either investigated. G The mRNA relative expression level of M1 phase is detected, including TNF-α, IL-1β, iNOS, and CD86. H The mRNA relative expression level of M2 phase is detected, including arginase 1, IL-10, and CD206. (* p < 0.05 versus LPS/IL-4, #p < 0.05 versus LPS/IL-4 + CD73+hucMSC-EVs, n = 3)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: Activation Assay, Expressing, Control

A 2b R knockdown cells and PKA inhibitor reverse the stimulatory effect of CD73+hucMSC-EVs. A – F BV2 cells are permanently transfected with scrambled shRNA or A 2b R shRNA. Cells are then treated with 1 μg/ml LPS or 5 μg/ml IL-4 in the presence or absence of 30 μg/ml CD73+ hucMSC-EVs. A and B Intracellular cAMP levels are measured after 10 min stimulation (n = 5). PKA protein expression is detected (n = 3). C and D The iNOS/Arg-1 protein expression in BV2 cells is investigated. E and F The mRNA relative expression level of M1/M2 phase is either detected. (* p < 0.05 versus shRNA scrambled/A 2b R + LPS, #p < 0.05 versus shRNA scrambled + CD73 + hucMSC-EVs). G and H The iNOS/Arg-1 protein expression in BV2 cells is investigated in presence or absence of 10 μM H-89 (PKA inhibitor)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: A 2b R knockdown cells and PKA inhibitor reverse the stimulatory effect of CD73+hucMSC-EVs. A – F BV2 cells are permanently transfected with scrambled shRNA or A 2b R shRNA. Cells are then treated with 1 μg/ml LPS or 5 μg/ml IL-4 in the presence or absence of 30 μg/ml CD73+ hucMSC-EVs. A and B Intracellular cAMP levels are measured after 10 min stimulation (n = 5). PKA protein expression is detected (n = 3). C and D The iNOS/Arg-1 protein expression in BV2 cells is investigated. E and F The mRNA relative expression level of M1/M2 phase is either detected. (* p < 0.05 versus shRNA scrambled/A 2b R + LPS, #p < 0.05 versus shRNA scrambled + CD73 + hucMSC-EVs). G and H The iNOS/Arg-1 protein expression in BV2 cells is investigated in presence or absence of 10 μM H-89 (PKA inhibitor)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: Knockdown, Transfection, shRNA, Expressing

CD73+hucMSC-EVs ameliorate SCI and decrease intracellular cAMP levels in mice. (A and B) In vivo biodistribution of DiR-labeled CD73+hucMSC-EVs. Mice are analyzed at 24 h after A subcutaneous injection of EVs around injured spinal cord and B intraperitoneal injection of EVs. C BMS scores at different time-point after spinal cord injury. D BBB scores at different time-point after spinal cord injury. E cAMP levels from cerebrospinal fluid at different time-point after spinal cord injury. F Histological images (H&E staing), G Nissl staining and (H) TUNEL staining of longitudinal sections of injured spinal cords on day 21

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: CD73+hucMSC-EVs ameliorate SCI and decrease intracellular cAMP levels in mice. (A and B) In vivo biodistribution of DiR-labeled CD73+hucMSC-EVs. Mice are analyzed at 24 h after A subcutaneous injection of EVs around injured spinal cord and B intraperitoneal injection of EVs. C BMS scores at different time-point after spinal cord injury. D BBB scores at different time-point after spinal cord injury. E cAMP levels from cerebrospinal fluid at different time-point after spinal cord injury. F Histological images (H&E staing), G Nissl staining and (H) TUNEL staining of longitudinal sections of injured spinal cords on day 21

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: In Vivo, Labeling, Injection, Staining, TUNEL Assay

CD73+hucMSC-EVs regulate M1/M2 polarization of microglia in mice. A and B Changes of arginase-1 and iNOS are determined by immunofluorescence in different groups at × 5 magnification. C Fluorescent intensities are normalized to the sham group. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI + CD73+hucMSC-EVs group, n = 5). D and E Representative dot spot of flow cytometry for microglia/macrophage subsets is shown. CD206 and CD86 are selected as biomarkers of M2 and M1 microglia, respectively. The data are calculated as M2:M1. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI + CD73+hucMSC-EVs group, n = 5)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: CD73+hucMSC-EVs regulate M1/M2 polarization of microglia in mice. A and B Changes of arginase-1 and iNOS are determined by immunofluorescence in different groups at × 5 magnification. C Fluorescent intensities are normalized to the sham group. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI + CD73+hucMSC-EVs group, n = 5). D and E Representative dot spot of flow cytometry for microglia/macrophage subsets is shown. CD206 and CD86 are selected as biomarkers of M2 and M1 microglia, respectively. The data are calculated as M2:M1. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI + CD73+hucMSC-EVs group, n = 5)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: Immunofluorescence, Flow Cytometry

CD73+hucMSC-EVs regulate the production of proinflammatory cytokines after SCI. A Cytokines of spinal samples from SCI mice are quantitative analyzed by Bio-Plex system 3d after SCI and hucMSC-EVs/CD73+hucMSC-EVs treatment. B – H The protein levels analyzed by Bio-Plex system, such as IL-1β, IL-6, TNF-α, IFN-γ, MCP-1 and MIP-1β are significantly decreased, while IL-4 and IL-10 are significantly increased. I – L The protein levels, including IL-1β, IL-6, TNF-α, and IL-10, are also analyzed by ELISA. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI+CD73+hucMSC-EVs group, n = 5)

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: CD73+hucMSC-EVs regulate the production of proinflammatory cytokines after SCI. A Cytokines of spinal samples from SCI mice are quantitative analyzed by Bio-Plex system 3d after SCI and hucMSC-EVs/CD73+hucMSC-EVs treatment. B – H The protein levels analyzed by Bio-Plex system, such as IL-1β, IL-6, TNF-α, IFN-γ, MCP-1 and MIP-1β are significantly decreased, while IL-4 and IL-10 are significantly increased. I – L The protein levels, including IL-1β, IL-6, TNF-α, and IL-10, are also analyzed by ELISA. (*p < 0.05 versus SCI group, #p < 0.05 versus SCI+CD73+hucMSC-EVs group, n = 5)

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: Enzyme-linked Immunosorbent Assay

Schematic diagram showing the effects of CD73+hucMSC-EVs on microglia

Journal: Journal of Nanobiotechnology

Article Title: Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

doi: 10.1186/s12951-021-01022-z

Figure Lengend Snippet: Schematic diagram showing the effects of CD73+hucMSC-EVs on microglia

Article Snippet: Besides, SCI mice were randomly divided into four groups, and 20 mice in each group were respectively treated with phosphate buffer saline (SCI group), or 20 μg hucMSC-EVs (SCI + hucMSC-EVs group), or 20 μg CD73 +hucMSC-EVs (SCI + CD73 +hucMSC-EVs group), or 20 ng rmCD73 (recombinant mouse CD73, Beijing Baiao Lai Bo Technology Co., Ltd., Beijing, China).

Techniques: